I decided to start another topic to keep track of the types of applications where the HiBiT tag is being used. Already seeing quite a bit of diversity: endogenous tagging, GPCR trafficking and virology.  Here are the ones I've seen so far.

1. Oh-hashi et al., 2017. Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells. Biochemistry and Biophysics Reports: http://www.sciencedirect.com/science/article/pii/S2405580817301644

The authors used CRISPR/Cas9 editing to tag endogenous ATF4 with HiBiT and then studied changes in abundance following various cell treatments.


 2. Schwinn et al., 2017. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chemical Biology. http://pubs.acs.org/doi/abs/10.1021/acschembio.7b00549.  

This publication is from Promega scientists that have performed endogenous HiBiT tagging to study various elements of the HIF1A signaling pathway.


3)Rouault et al, 2017. Regions of MRAP2 required for the inhibition of orexin and prokineticin receptor signaling. BBA-Molecular Cell Research http://www.sciencedirect.com/science/article/pii/S0167488917302513

 Authors demonstrate use of HiBiT Extracellular system to study the role of MRAP2 in GPCR trafficking and surface expression.


4) Sasaki et al., 2017. Development of a rapid and quantitative method for the analysis of viral entry and release using a NanoLuc luciferase complementation assay. Virus Research


The authors developed quantitative methods for the detection of cellular entry and release of subviral and flavivirus-like particles (SVPs, VLPs) by HiBiT tagging the particles. The HiBiT tag was used for quantitation of the particles and cellular entry was studied using a LgBiT expressing stable Vero cell line.