I ran out of the passive lysis buffer (E1941), hence I debated whether I could have completed my luci assay (E1501) if I had made any standard SDS lysis buffer instead. However, I read that the lysis buffer could introduce variability from autoluminescence (pasted below). Would that have been the case? Thanks much in advance!
For the Dual-Luciferase® Reporter Assay System, only use Passive Lysis Buffer since PLB is specially formulated to minimize background autoluminescence.