To learn more visit: www.Promega.com/HiBiT
In contrast to the low affinity interaction between LgBiT and SmBiT used to study protein interactions, the NanoBiT system was also developed with a small peptide (HiBiT) that has high affinity for the LgBiT subunit. The 11 aa HiBiT peptide interacts spontaneously with LgBiT to give a bright luminescent enzyme. Promega is currently developing products based on this high affinity interaction including 1) lytic detection reagents for the HiBiT tag; 2) live cell reagents to detect HiBiT on surface-expressed or secreted proteins; 3) HiBiT blotting reagents that enable blots to be performed in just a few minutes; and 4) live cell detection of the intracellular HiBiT:LgBiT interaction.
Would you like to learn more or become and early evaluator? We currently have test materials available. Please leave a comment to indicate your interest and we’ll get back to you.